D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant Q124C

Expression Data

Yield (mg/mL): 1.32

Kinetic Assay Data

**slope of Q124C A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	6.96E-02	7.64E-02	8.20E-02
B	25.00	6.14E-02	6.57E-02	6.86E-02
C	8.33	0	0	3.00E-04
D	2.78
E	0.93
F	0.31
G	0.10
H	0.03

Yield (mg/mL): 1.32 Dilution Factor: 100×

Protein purified on 11/2/22 and assayed on 11/9/22.
Data uploaded by gabefletes and last updated on 2022-11-18 18:18:25.

Thermostability Assay Data

**slope of Q124C A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	6.00E-04	0	0
B	48.3	4.00E-04	7.00E-04	0
C	45.7	5.00E-04	0	4.00E-04
D	42.4		7.00E-04	8.00E-04
E	37.7	1.57E-02	1.23E-02	1.34E-02
F	33.6	2.14E-02	2.06E-02
G	31.3	2.70E-02	3.13E-02	2.58E-02
H	30.0	2.40E-02	2.47E-02	2.53E-02

Protein purified on 11/2/22 and assayed on 11/9/22.
Data uploaded by gabefletes and last updated on 2022-11-18 18:23:25.

Design Data

Rosetta Score for Q124C: -1089.7

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.