D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant I90V

Yield (mg/mL): 1.252

**slope of I90V A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.119397	0.127374	0.123956
B	18.75	0.094543	0.100096	0.0935127
C	4.69	0.0541091	0.0573345	0.0537927
D	1.17	0.0211909	0.0224976	0.021697
E	0.29	0.00621152	0.00667455	0.00667394
F	0.07	0.0017503	0.00217636	0.00178121
G	0.02	0.000470303	0.00052	0.000486061
H	0.00		0.000227273	2.78E-04

Yield / A_{280 nm}: 1.252 Dilution Factor: 100×

Protein purified on 9/21/19 and assayed on 9/21/19.
Data uploaded by peishanh and last updated on 2022-06-27 07:04:03.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.83E-02	3.83E-02	3.67E-02
B	18.75	2.87E-02	2.90E-02	2.75E-02
C	4.69	1.81E-02	1.85E-02	1.75E-02
D	1.17	8.66E-03	7.97E-03	6.91E-03
E	0.29	2.80E-03	2.45E-03	2.24E-03
F	0.07	8.14E-04	6.82E-04	6.20E-04
G	0.02	2.75E-04	2.06E-04	2.08E-04
H	0.00	8.12E-05	6.67E-05	7.21E-05

Yield / A_{280 nm}: 0.207 Dilution Factor: 50×

Protein purified on 9/21/19 and assayed on 9/21/19.
Data uploaded by peishanh and last updated on 2022-06-27 07:04:03.

The above data have been published in the following journal article(s):

• Huang, P.; Chu, S.K.; Frizzo, H.N.; Connolly, M.P.; Caster, R.W.; & Siegel, J. B. (2020) “Evaluating Protein Engineering Thermostability Prediction Tools Using an Independently Generated Dataset..” ACS Omega 5(12), 6487–6493.