Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.56

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.05005	0.05545	0.0509
	B	25.00	0.0451	0.05685	0.0494
	C	8.33	0.04285	0.0428	0.041
	D	2.78	0.01635	0.01995	0.0179
	E	0.93	0.0095	0.008151	0.00845
	F	0.31	0.00395	0.0033	0.00235
	G	0.10		0.0014	0.00125
	H	0.03	0	0.0002	0.00035

Yield (mg/mL): 0.56     Dilution Factor:  100×

Download these data

Protein purified on 11/2/2022 and assayed on 11/9/2022.
Data uploaded by jannahal and last updated on 2022-11-18 19:11:00.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0	0	0
	B	48.3	0	0	0
	C	45.7	0	0.0007	0.0006
	D	42.4	0.0019	0.0021
	E	37.7	0.0124	0.0135	0.0109
	F	33.6	0.012	0.0149	0.0157
	G	31.3	0.0101	0.013	0.0164
	H	30.0		0.0084	0.0085

Download these data

Protein purified on 11/2/2022 and assayed on 11/9/2022.
Data uploaded by jannahal and last updated on 2022-11-18 19:16:13.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.