Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.24

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	1.144	1.299	1.217
	B	25.00	1.081	0.9977	1.03
	C	8.33
	D	2.78	0.6354	0.6415	0.5469
	E	0.93	0.3863	0.3417	0.3445
	F	0.31	0.1713	0.1496	0.1653
	G	0.10	0.0614	0.05525	0.06386
	H	0.03	0.0212	0.0189	0.02085

Yield (mg/mL): 1.24     Dilution Factor:  10×

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Protein purified on 11/9/22 and assayed on 11/9/22.
Data uploaded by vater and last updated on 2022-11-21 11:20:09.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	4.00E-04	3.00E-04	6.00E-04
	B	48.3		4.00E-04	4.00E-04
	C	45.7	8.00E-04		1.00E-03
	D	42.4	3.43E-02	3.54E-02
	E	37.7	7.22E-01	6.56E-01	5.70E-01
	F	33.6	7.10E-01	6.47E-01	6.67E-01
	G	31.3	7.51E-01	6.51E-01	6.71E-01
	H	30.0	6.51E-01	6.56E-01	6.18E-01

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Protein purified on and assayed on .
Data uploaded by Jsolon and last updated on 2022-11-21 23:06:52.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.