Raw Data for BglB Variant H194V

Expression Data

SDS-PAGE
 1.04

Kinetic Assay Data

slope of H194V A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.05635 0.05630 0.05560
B 25.00 0.04415 0.04870 0.05445
C 8.33 0.04960 0.04630 0.04040
D 2.78 0.02140 0.02170 0.02210
E 0.93 0.007700 0.008100 0.006601
F 0.31 0.003100 0.003000 0.002600
G 0.10 0.001350 0.001250 0.001350
H 0.03 0.0003500 0.0003000 0.0002500
 1.04      100×

Protein purified on 11/2/2022 and assayed on 11/9/2022.
Data uploaded by nsclin and last updated on 2022-11-29 16:57:52.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.05005 0.05545 0.0509
B 25.00 0.0451 0.05685 0.0494
C 8.33 0.04285 0.0428 0.041
D 2.78 0.01635 0.01995 0.0179
E 0.93 0.0095 0.008151 0.00845
F 0.31 0.00395 0.0033 0.00235
G 0.10 0.0014 0.00125
H 0.03 0 0.0002 0.00035
 0.56      100×

Protein purified on 11/2/2022 and assayed on 11/9/2022.
Data uploaded by jannahal and last updated on 2022-11-18 19:11:00.


Thermostability Assay Data

slope of H194V A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0 6.00E-04
B 48.3 0 6.00E-04 0
C 45.7 8.00E-04 5.00E-04 0
D 42.4 9.00E-04 7.00E-04 8.00E-04
E 37.7 2.10E-03 2.30E-03 1.70E-03
F 33.6 2.70E-03 2.50E-03 3.10E-03
G 31.3 3.00E-03 2.30E-03 2.40E-03
H 30.0 2.40E-03 2.70E-03 2.50E-03

Protein purified on 11/2/2022 and assayed on 11/9/2022.
Data uploaded by nsclin and last updated on 2022-11-29 17:02:25.


Reference Data for WT

slope of H194V A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0 0
B 48.3 0 0 0
C 45.7 0 0.0007 0.0006
D 42.4 0.0019 0.0021
E 37.7 0.0124 0.0135 0.0109
F 33.6 0.012 0.0149 0.0157
G 31.3 0.0101 0.013 0.0164
H 30.0 0.0084 0.0085

Protein purified on 11/2/2022 and assayed on 11/9/2022.
Data uploaded by jannahal and last updated on 2022-11-18 19:16:13.


Design Data

22.145

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.