Raw Data for BglB Variant T175A

Expression Data

Kinetic Assay Data

Plate 20201217-1

slope of T175A A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.0200467	0.020037	0.0185776
	B	18.75	0.0134758	0.0131291	0.0130024
	C	4.69	0.00733636	0.00665515	0.00707758
	D	1.17	0.00312242	0.00289394	0.00301939
	E	0.29		0.000640606	0.000600606
	F	0.07	0.000246667	0.00022303	0.00024
	G	0.02	8.48E-05	7.21E-05	6.42E-05
	H	0.00		0	0

Yield / A_{280 nm}: 0.383     Dilution Factor:  100×

Download these data

Protein purified on 9/21/19 and assayed on 9/21/19.
Data uploaded by peishanh and last updated on 2022-06-27 07:04:03.

Reference Data for WT

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	3.83E-02	3.83E-02	3.67E-02
	B	18.75	2.87E-02	2.90E-02	2.75E-02
	C	4.69	1.81E-02	1.85E-02	1.75E-02
	D	1.17	8.66E-03	7.97E-03	6.91E-03
	E	0.29	2.80E-03	2.45E-03	2.24E-03
	F	0.07	8.14E-04	6.82E-04	6.20E-04
	G	0.02	2.75E-04	2.06E-04	2.08E-04
	H	0.00	8.12E-05	6.67E-05	7.21E-05

Yield / A_{280 nm}: 0.207     Dilution Factor:  50×

Download these data

Protein purified on 9/21/19 and assayed on 9/21/19.
Data uploaded by peishanh and last updated on 2022-06-27 07:04:03.

Citations

The above data have been published in the following journal article(s):

• • Huang, P.; Chu, S.K.; Frizzo, H.N.; Connolly, M.P.; Caster, R.W.; & Siegel, J. B. (2020) “Evaluating Protein Engineering Thermostability Prediction Tools Using an Independently Generated Dataset..” ACS Omega 5(12), 6487–6493.