Raw Data for BglB Variant F274N

Expression Data

SDS-PAGE
 0.13

Kinetic Assay Data

slope of F274N A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.20E+02 2.44E+02 2.92E+02
B 25.00 2.11E+02 2.30E+02 2.88E+02
C 8.33 1.90E+02 1.74E+02 1.89E+02
D 2.78 9.60E+01 9.17E+01 1.28E+02
E 0.93 4.20E+01 3.57E+01 3.69E+01
F 0.31 1.29E+01 1.54E+01
G 0.10 5.33E+00 4.99E+00
H 0.03 1.40E-01 2.70E-01 4.00E-02
 0.13      10×

Protein purified on 11/3/2022 and assayed on 11/17/2022.
Data uploaded by esteffen27 and last updated on 2022-12-08 13:09:08.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 9.64E+00 1.03E+01
B 25.00 7.48E+00 8.40E+00 8.25E+00
C 8.33 4.49E+00 4.51E+00
D 2.78 3.50E+00 3.97E+00
E 0.93 1.23E+00 1.15E+00
F 0.31 5.30E-01 5.10E-01 5.30E-01
G 0.10 3.80E-01 1.80E-01 3.00E-01
H 0.03 2.40E-01 2.48E-01 2.60E-01
 0.382      10×

Protein purified on 11/3/2022 and assayed on 11/17/2022.
Data uploaded by esteffen27 and last updated on 2023-12-20 14:45:09.



Design Data

6.725


Citations

The unpublished data above were collected by students from Crown College. For inquiries, please contact Aeisha Thomas: <thomasa@crown.edu>.