D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W409Y

Expression Data

Yield (mg/mL): 0.258663

Kinetic Assay Data

**slope of W409Y A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00	4.31E-02	4.74E-02	5.87E-02
C	8.33	4.11E-02	3.92E-02	4.23E-02
D	2.78	2.71E-02	2.62E-02	2.75E-02
E	0.93	1.31E-02	1.33E-02	1.28E-02
F	0.31	5.30E-03	5.25E-03	5.20E-03
G	0.10	1.90E-03	1.95E-03	1.80E-03
H	0.03	2.00E-04	1.50E-04	2.00E-04

Yield (mg/mL): 0.258663 Dilution Factor: 100×

Protein purified on 11/9/2022 and assayed on 11/16/2022.
Data uploaded by DanielleBeverSneary and last updated on 2022-12-12 11:43:54.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00	2.91E-02	2.85E-02	2.88E-02
C	8.33	2.31E-02	2.25E-02	2.32E-02
D	2.78	1.41E-02	1.39E-02	1.40E-02
E	0.93	6.55E-03	6.35E-03	6.30E-03
F	0.31	2.60E-03	2.40E-03	2.40E-03
G	0.10	1.05E-03	1.00E-03	9.50E-04
H	0.03	2.00E-04	1.50E-04	2.50E-04

Yield (mg/mL): 0.310937 Dilution Factor: 100×

Protein purified on 11/9/2022 and assayed on 11/16/2022.
Data uploaded by DanielleBeverSneary and last updated on 2022-12-12 09:19:22.

Design Data

Rosetta Score for W409Y: 0.36

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Danielle BeverSneary: <dbeversneary@miracosta.edu>.