D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant T13S

Expression Data

Yield (mg/mL): 0.0986099

Kinetic Assay Data

**slope of T13S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00	1.07E-02	1.05E-02	1.10E-02
C	8.33	8.95E-03	9.60E-03	9.65E-03
D	2.78	6.60E-03	6.55E-03	5.95E-03
E	0.93	2.85E-03	2.70E-03	3.15E-03
F	0.31	1.30E-03	1.15E-03	1.20E-03
G	0.10	5.00E-04	5.00E-04	5.50E-04
H	0.03	1.50E-04	2.50E-04	2.00E-04

Yield (mg/mL): 0.0986099 Dilution Factor: 100×

Protein purified on 11/9/2022 and assayed on 11/16/2022.
Data uploaded by DanielleBeverSneary and last updated on 2022-12-12 12:33:24.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00	2.91E-02	2.85E-02	2.88E-02
C	8.33	2.31E-02	2.25E-02	2.32E-02
D	2.78	1.41E-02	1.39E-02	1.40E-02
E	0.93	6.55E-03	6.35E-03	6.30E-03
F	0.31	2.60E-03	2.40E-03	2.40E-03
G	0.10	1.05E-03	1.00E-03	9.50E-04
H	0.03	2.00E-04	1.50E-04	2.50E-04

Yield (mg/mL): 0.310937 Dilution Factor: 100×

Protein purified on 11/9/2022 and assayed on 11/16/2022.
Data uploaded by DanielleBeverSneary and last updated on 2022-12-12 09:19:22.

Design Data

Rosetta Score for T13S: 2.808

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Danielle BeverSneary: <dbeversneary@miracosta.edu>.