D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W325M

Expression Data

Yield (mg/mL): 1.67

Kinetic Assay Data

**slope of W325M A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.82E-02	2.17E-02	1.91E-02
B	25.00	1.42E-02	2.04E-02	2.03E-02
C	8.33	1.55E-02	1.41E-02	1.27E-02
D	2.78		7.05E-03	6.75E-03
E	0.93	3.40E-03	3.20E-03	3.10E-03
F	0.31	1.30E-03	1.20E-03	1.20E-03
G	0.10	5.00E-04	5.50E-04	5.50E-04
H	0.03	3.50E-04	3.50E-04	0

Yield (mg/mL): 1.67 Dilution Factor: 100×

Protein purified on 11/3/2022 and assayed on 11/4/2022.
Data uploaded by anng19 and last updated on 2023-01-30 16:54:47.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		2.35E-01	2.15E-01
B	25.00		1.37E-01	1.52E-01
C	8.33	6.51E-02	1.55E-01	1.32E-01
D	2.78	4.51E-02	7.62E-02	5.99E-02
E	0.93		2.02E-02	2.17E-02
F	0.31	1.10E-02	3.25E-03	7.40E-03
G	0.10		3.35E-03	2.70E-03
H	0.03		4.50E-04	4.50E-04

Yield (mg/mL): 1.842 Dilution Factor: 100×

Protein purified on 11/3/2022 and assayed on 11/4/2022.
Data uploaded by anng19 and last updated on 2023-01-30 16:58:09.

Design Data

Rosetta Score for W325M: 3.455

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.