D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N404M

Expression Data

Yield (mg/mL): 0.576

Kinetic Assay Data

**slope of N404M A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.009600	0.01055	0.01100
B	25.00	0.007400	0.008300	0.008150
C	8.33	0.004750	0.004150	0.004900
D	2.78	0.001950	0.001850	0.001950
E	0.93	0.0008500	0.0007500	0.0008000
F	0.31		0.0005500	0.0005000
G	0.10	0.0002500	0.0000000	0.0003500
H	0.03	0.0002500	0.0002500	0

Yield (mg/mL): 0.576 Dilution Factor: 10×

Protein purified on 2/28/23 and assayed on 3/1/23.
Data uploaded by anarsam and last updated on 2023-03-24 02:02:39.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2389	0.2363	0.2376
B	25.00	0.2200	0.2289	0.2490
C	8.33	0.1705	0.1842	0.1862
D	2.78	0.09585	0.1003	0.1156
E	0.93	0.02575	0.03910	0.04405
F	0.31	0.01675	0.01690	0.01680
G	0.10	0.005650	0.005600	0.005450
H	0.03	0.0003000	0.0003000	0

Yield (mg/mL): 2.532 Dilution Factor: 100×

Protein purified on 2/28/23 and assayed on 3/1/23.
Data uploaded by anarsam and last updated on 2023-03-23 14:13:16.

Design Data

Rosetta Score for N404M: -3.039

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.