Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.1449

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.5	0.46	0.43
	B	25.00	0.42	0.41	0.41
	C	8.33	0.32	0.28	0.31
	D	2.78	0.11	0.11	0.1
	E	0.93	0.04	0.04	0.04
	F	0.31		0.003351	0.001809
	G	0.10
	H	0.03	0.002314	0.002057	0.001543

Yield (mg/mL): 1.1449     Dilution Factor:  100×

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Protein purified on 2/17/2023 and assayed on 3/3/2023.
Data uploaded by enielse and last updated on 2023-03-28 11:10:34.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.001946		0.002203
	B	48.3	0.0012	0.001303
	C	45.7		0.003471	0.003591
	D	42.4	0.03	0.03	0.03
	E	37.7
	F	33.6	0.09	0.08	0.07
	G	31.3
	H	30.0	0.11	0.12	0.11

Download these data

Protein purified on 2/17/2023 and assayed on 3/17/2023.
Data uploaded by enielse and last updated on 2023-03-28 11:13:35.

Citations

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.