Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.1449

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.36	0.35	0.35
	B	25.00	0.22	0.21	0.2
	C	8.33	0.11	0.11
	D	2.78	0.11	0.11
	E	0.93	0.03	0.03	0.03
	F	0.31	0.01	0.01	0.01
	G	0.10	0.006189	0.006129	0.006197
	H	0.03		0.00252	0.002477

Yield (mg/mL): 1.1449     Dilution Factor:  100×

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Protein purified on 2/17/2023 and assayed on 3/3/2023.
Data uploaded by enielse and last updated on 2023-03-29 06:21:00.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.001251	0.002511	0.003
	B	48.3	0.002846		0.002211
	C	45.7
	D	42.4	0.03	0.03	0.03
	E	37.7		0.1	0.1
	F	33.6	0.13	0.13
	G	31.3	0.13	0.14
	H	30.0	0.17	0.18

Download these data

Protein purified on 2/17/2023 and assayed on 3/17/2023.
Data uploaded by enielse and last updated on 2023-03-29 06:33:53.

Citations

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.