D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant F253I

Expression Data

Yield (mg/mL): 1.252

Kinetic Assay Data

**slope of F253I A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.49E-01	1.56E-01	1.69E-01
B	25.00	1.16E-01	1.19E-01	1.24E-01
C	8.33	5.04E-02	4.85E-02	5.24E-02
D	2.78	1.24E-02	1.22E-02	1.40E-02
E	0.93	2.45E-03	2.80E-03	2.80E-03
F	0.31	6.50E-04	7.50E-04	7.00E-04
G	0.10	0	0	0
H	0.03	0	0	0

Yield (mg/mL): 1.252 Dilution Factor: 10×

Protein purified on 2/1/2023 and assayed on 2/3/2023.
Data uploaded by sdas407 and last updated on 2024-08-12 16:35:32.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.96E-01	2.06E-01	2.04E-01
B	25.00	1.19E-01	1.65E-01	1.61E-01
C	8.33	8.26E-02	7.93E-02	7.38E-02
D	2.78	2.40E-02	2.37E-02	2.35E-02
E	0.93	5.50E-03	5.60E-03	5.55E-03
F	0.31	1.65E-03	1.65E-03	1.65E-03
G	0.10	6.00E-04	7.50E-04	5.50E-04
H	0.03	0	0

Yield (mg/mL): 2.203 Dilution Factor: 100×

Protein purified on 2/1/23 and assayed on 2/3/23.
Data uploaded by sdas407 and last updated on 2025-02-11 18:49:36.

Design Data

Rosetta Score for F253I: 6.605

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.