D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant T13S

Yield (mg/mL): 0.659

**slope of T13S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.30E-01	3.40E-01	3.50E-01
B	25.00	2.00E-01	2.20E-01	2.30E-01
C	8.33	1.00E-01	9.00E-02
D	2.78	4.00E-02	5.00E-02	4.00E-02
E	0.93	1.00E-02	1.00E-02	1.00E-02
F	0.31	4.23E-03	4.56E-03	4.94E-03
G	0.10		3.44E-03	3.51E-03
H	0.03

Yield (mg/mL): 0.659 Dilution Factor: 10×

Protein purified on 2/17/23 and assayed on 3/17/23.
Data uploaded by timeaboth and last updated on 2023-03-31 08:41:45.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.48	0.45	0.44
B	25.00	0.4	0.44	0.39
C	8.33	0.34	0.31	0.28
D	2.78	0.11	0.1	0.1
E	0.93	0.04	0.04	0.04
F	0.31	0.02	0.02	0.02
G	0.10	0.006077	0.006857	0.008169
H	0.03	0.003531	0.001286	0.001903

Yield (mg/mL): 1.1449 Dilution Factor: 100×

Protein purified on 2/17/2023 and assayed on 3/3/2023.
Data uploaded by enielse and last updated on 2023-03-28 10:55:15.

**slope of T13S A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	2.42E-03	2.61E-03	2.95E-03
B	48.3	1.81E-03	2.21E-03	1.78E-03
C	45.7	2.07E-03	2.61E-03	1.05E-03
D	42.4		1.00E-02	8.29E-03
E	37.7	5.80E-01	6.60E-01	6.30E-01
F	33.6	7.20E-01	7.50E-01	7.10E-01
G	31.3	7.10E-01	7.40E-01	8.00E-01
H	30.0	5.80E-01	7.50E-01	6.60E-01

Protein purified on 2/17/2023 and assayed on 3/17/2023.
Data uploaded by timeaboth and last updated on 2023-03-31 08:34:48.

**slope of T13S A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0.002134	0.002443	0.001466
B	48.3	0.001817		0.002469
C	45.7	0.003857	0.003677
D	42.4	0.02	0.02
E	37.7	0.07	0.1
F	33.6	0.12	0.12	0.1
G	31.3	0.09	0.12	0.14
H	30.0		0.13	0.14

Protein purified on 2/17/2023 and assayed on 3/17/2023.
Data uploaded by enielse and last updated on 2023-03-28 11:02:34.

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.