Raw Data for BglB Variant H328N

Expression Data

SDS-PAGE
 0.636

Kinetic Assay Data

slope of H328N A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 6.30E-01 5.60E-01 6.20E-01
B 25.00 5.20E-01 6.10E-01
C 8.33
D 2.78 7.20E-01 7.50E-01
E 0.93 3.80E-01 3.20E-01
F 0.31 1.95E-03 2.36E-03
G 0.10
H 0.03 2.15E-03 2.43E-03 2.63E-03
 0.636      100×

Protein purified on 2/17/23 and assayed on 3/3/23.
Data uploaded by cmartin13 and last updated on 2023-03-31 08:02:42.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.5 0.46 0.43
B 25.00 0.42 0.41 0.41
C 8.33 0.32 0.28 0.31
D 2.78 0.11 0.11 0.1
E 0.93 0.04 0.04 0.04
F 0.31 0.003351 0.001809
G 0.10
H 0.03 0.002314 0.002057 0.001543
 1.1449      100×

Protein purified on 2/17/2023 and assayed on 3/3/2023.
Data uploaded by enielse and last updated on 2023-03-28 11:10:34.


Thermostability Assay Data

slope of H328N A420 nm (min−1)
T (°C) 1 2 3
A 50.0 1.08E-03 2.59E-03 1.70E-03
B 48.3 1.70E-03 1.39E-03
C 45.7 1.41E-03 1.64E-03 1.59E-03
D 42.4 6.70E-03 5.31E-03 4.01E-03
E 37.7 2.00E-02 2.00E-02 2.00E-02
F 33.6 4.00E-02 4.00E-02 4.00E-02
G 31.3 3.00E-02 5.00E-02 2.00E-02
H 30.0 3.00E-02 3.00E-02

Protein purified on 2/17/23 and assayed on 3/17/23.
Data uploaded by cmartin13 and last updated on 2023-03-31 08:15:48.


Reference Data for WT

slope of H328N A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.001946 0.002203
B 48.3 0.0012 0.001303
C 45.7 0.003471 0.003591
D 42.4 0.03 0.03 0.03
E 37.7
F 33.6 0.09 0.08 0.07
G 31.3
H 30.0 0.11 0.12 0.11

Protein purified on 2/17/2023 and assayed on 3/17/2023.
Data uploaded by enielse and last updated on 2023-03-28 11:13:35.


Design Data

0.622

Citations

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.