D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant A80S

Yield (mg/mL): 0.0567

**slope of A80S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.01	0.01	0.01
B	25.00	0.009754	0.009489	0.009831
C	8.33	0.007131	0.007474	0.007809
D	2.78	0.006189	0.005769	0.004731
E	0.93	0.003977	0.00438	0.003249
F	0.31	0.003111		0.002906
G	0.10
H	0.03		0.001834	0.001877

Yield (mg/mL): 0.0567 Dilution Factor: 100×

Protein purified on 2/17/2023 and assayed on 3/3/2023.
Data uploaded by VNikolovski and last updated on 2023-03-31 10:50:50.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.35	0.36	0.31
B	25.00	0.19	0.17	0.17
C	8.33	0.1	0.09	0.09
D	2.78	0.05	0.05	0.05
E	0.93		0.02	0.02
F	0.31	0.01	0.01	0.009849
G	0.10	0.0054	0.005871	0.005006
H	0.03	0.003069	0.002743	0.00336

Yield (mg/mL): 1.1449 Dilution Factor: 100×

Protein purified on 2/17/2023 and assayed on 3/3/2023.
Data uploaded by enielse and last updated on 2023-03-29 05:56:41.

**slope of A80S A_{420 nm} (min⁻¹)**
		T (°C)	1	2	3
	A	30.0	3.87E-03	2.57E-03	3.13E-03
	B