Raw Data for BglB Variant H178C

Expression Data

SDS-PAGE
 0.037

Kinetic Assay Data

slope of H178C A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 8.12E-03 8.34E-03 8.81E-03
B 25.00
C 8.33 7.82E-03 6.87E-03 6.85E-03
D 2.78 5.37E-03 5.13E-03 5.05E-03
E 0.93 3.45E-03 4.42E-03 3.32E-03
F 0.31 2.96E-03 2.25E-03
G 0.10 2.48E-03 2.79E-03
H 0.03 2.49E-03 2.73E-03
 0.037      10×

Protein purified on 2/17/23 and assayed on 3/3/23.
Data uploaded by nalejandro and last updated on 2023-03-31 10:59:59.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.35 0.31 0.34
B 25.00 0.22 0.19 0.18
C 8.33 0.1 0.1 0.1
D 2.78 0.06 0.06 0.06
E 0.93 0.03 0.03 0.03
F 0.31 0.01 0.01 0.01
G 0.10 0.005486 0.005614 0.00618
H 0.03 0.002563 0.002169 0.002263
 1.1449      100×

Protein purified on 2/17/2023 and assayed on 3/3/2023.
Data uploaded by enielse and last updated on 2023-03-29 06:07:28.


Thermostability Assay Data

Data missing or deleted from the database.


Reference Data for WT

slope of H178C A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.002614 0.002126
B 48.3 0.002391 0.00234 0.002126
C 45.7 0.003643 0.003369
D 42.4 0.03 0.03
E 37.7 0.1 0.11
F 33.6 0.1 0.1 0.11
G 31.3 0.14 0.14 0.15
H 30.0 0.15 0.12 0.13

Protein purified on 2/17/2023 and assayed on 3/17/2023.
Data uploaded by enielse and last updated on 2023-03-29 06:11:52.


Design Data

3.459

Citations

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.