D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H119D

Expression Data

Yield (mg/mL): 0.642

Kinetic Assay Data

**slope of H119D A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.06E-02	5.18E-02	5.07E-02
B	25.00	3.42E-02	3.38E-02	3.32E-02
C	8.33	1.56E-02	1.55E-02	1.58E-02
D	2.78	4.60E-03	4.30E-03	4.30E-03
E	0.93	1.10E-03	1.05E-03	1.30E-03
F	0.31	4.50E-04	5.00E-04	4.00E-04
G	0.10	4.00E-04	3.50E-04	4.00E-04
H	0.03	2.50E-04	2.00E-04	0

Yield (mg/mL): 0.642 Dilution Factor: 10×

Protein purified on 2/1/2023 and assayed on 2/3/2023.
Data uploaded by sdas407 and last updated on 2023-04-10 12:57:38.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.38E-01	1.74E-01	2.21E-01
B	25.00	1.67E-01	1.62E-01	1.61E-01
C	8.33	8.75E-02	8.53E-02	8.56E-02
D	2.78	2.65E-02	2.75E-02	2.76E-02
E	0.93	6.30E-03	6.30E-03	6.15E-03
F	0.31	1.50E-03	1.75E-03	1.45E-03
G	0.10	4.00E-04	6.50E-04	5.50E-04
H	0.03	0	0	3.50E-04

Yield (mg/mL): 2.079 Dilution Factor: 10×

Protein purified on 2/1/2023 and assayed on 2/3/2023.
Data uploaded by sdas407 and last updated on 2023-04-10 12:53:41.

Design Data

Rosetta Score for H119D: 3.203

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.