Raw Data for BglB Variant N157Y

Expression Data

Yield (mg/mL): 1.15

Kinetic Assay Data

slope of N157Y A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	4.61E-01	5.09E-01	5.21E-01
	B	25.00	3.50E-01	3.57E-01	3.54E-01
	C	8.33	2.60E-01		2.92E-01
	D	2.78	1.55E-01	1.60E-01	1.68E-01
	E	0.93	6.65E-02	5.83E-02
	F	0.31	3.04E-02	2.62E-02	3.16E-02
	G	0.10	1.04E-02	9.78E-03	1.25E-02
	H	0.03	3.65E-03	0	5.74E-03

Yield (mg/mL): 1.15     Dilution Factor:  100×

Download these data

Protein purified on 3/9/2023 and assayed on 3/30/2023.
Data uploaded by nehemiahrao and last updated on 2023-04-13 11:53:18.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Thermostability Assay Data

slope of N157Y A_{420 nm} (min⁻¹)

		T (°C)	1	2
	A	30.0	1.90E-01	2.21E-01
	B	30.5	2.04E-01	2.01E-01
	C	31.7	1.82E-01	1.92E-01
	D	33.2	0.19086	0.18734
	E	35.4	0.20183	0.19086
	F	38.3	0.16655	0.17059
	G	41.7	0.091966	0.08431
	H	44.5	0.0062069	0.0057931
	I	46.8	0.0018621	0.0013448
	J	48.3	0.0012203	0.0017586
	K	49.5	0	0.0019322
	L	50.0	0.0010345	0

Download these data

Protein purified on 3/9/2023 and assayed on 4/4/2023.
Data uploaded by nehemiahrao and last updated on 2023-04-13 12:09:11.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Design Data

Citations

The unpublished data above were collected by students from Taylor University. For inquiries, please contact Daniel Kaluka: <daniel_kaluka@taylor.edu>.