Raw Data for BglB Variant Q124G

Expression Data

SDS-PAGE
 0.245

Kinetic Assay Data

slope of Q124G A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 4.75E-02 4.80E-02 5.06E-02
B 25.00 4.16E-02 4.03E-02 3.96E-02
C 8.33 3.05E-02 2.92E-02 2.85E-02
D 2.78 1.71E-02 1.62E-02 1.63E-02
E 0.93 7.95E-03 7.65E-03 6.75E-03
F 0.31 3.05E-03 2.75E-03 2.35E-03
G 0.10 1.20E-03 1.00E-03
H 0.03 0 2.50E-04 2.50E-04
 0.245      10×

Protein purified on 3/30/2023 and assayed on 3/31/2023.
Data uploaded by marisa975730 and last updated on 2023-04-13 10:22:38.



Thermostability Assay Data

slope of Q124G A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0 6.00E-04
B 48.8 4.00E-04 6.00E-04 7.00E-04
C 46.5 4.00E-04 0 7.00E-04
D 42.5 1.40E-03 1.30E-03 1.60E-03
E 37.6 6.60E-03 6.10E-03
F 33.8 1.26E-02 1.26E-02 1.09E-02
G 31.2 1.56E-02 1.64E-02 1.48E-02
H 30.0 1.70E-02 1.41E-02 1.49E-02

Protein purified on 3/30/2023 and assayed on 4/6/2023.
Data uploaded by marisa975730 and last updated on 2023-04-13 10:46:54.


Reference Data for WT

slope of Q124G A420 nm (min−1)
T (°C) 1 2 3
A 30.0 8.00E-04 3.60E-03
B