Raw Data for BglB Variant H194L

Expression Data

SDS-PAGE
 0.29

Kinetic Assay Data

slope of H194L A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.01E-02 1.07E-02 1.10E-02
B 25.00 7.80E-03 9.55E-03 1.10E-02
C 8.33 8.05E-03 8.70E-03 8.15E-03
D 2.78 4.50E-03 4.65E-03 4.10E-03
E 0.93 1.85E-03 1.80E-03 1.85E-03
F 0.31 8.00E-04 8.00E-04 7.50E-04
G 0.10 3.50E-04 3.50E-04 3.50E-04
H 0.03 0 0 0
 0.29      100×

Protein purified on 3/30/2023 and assayed on 3/31/2023.
Data uploaded by VongphakdyK and last updated on 2023-04-13 10:21:21.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 5.03E-02 5.31E-02 4.80E-02
B 25.00 4.16E-02 4.57E-02 4.38E-02
C 8.33 3.86E-02 4.19E-02 3.60E-02
D 2.78 1.86E-02 1.93E-02 2.07E-02
E 0.93 9.05E-03 9.80E-03 7.35E-03
F 0.31 3.55E-03 3.60E-03 3.65E-03
G 0.10 1.65E-03 1.85E-03 1.50E-03
H 0.03 0 1.00E-04 1.50E-04
 1.01      100×

Protein purified on 3/30/2023 and assayed on 3/31/2023.
Data uploaded by VongphakdyK and last updated on 2023-04-13 10:36:47.


Thermostability Assay Data

slope of H194L A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0 6.00E-04
B 48.8 0 0 0
C 46.5 6.00E-04 5.00E-04 0
D 42.5 3.90E-03 4.00E-03
E 37.6 3.72E-02 3.59E-02 2.86E-02
F 33.8 5.53E-02 6.36E-02 5.56E-02
G 31.7 6.16E-02 7.01E-02 6.28E-02
H 30.0 5.87E-02 6.37E-02 7.43E-02

Protein purified on 3/30/2023 and assayed on 4/6/2023.
Data uploaded by VongphakdyK and last updated on 2023-04-13 10:46:30.


Reference Data for WT

slope of H194L A420 nm (min−1)
T (°C) 1 2 3
A 50.0 7.00E-04 4.00E-04
B 48.8 0 8.00E-04 4.00E-04
C 46.5 1.30E-03 1.30E-03 1.60E-03
D 42.5 1.16E-01 7.51E-02 9.32E-02
E 37.6 2.90E-01 2.75E-01 3.32E-01
F 33.8 2.51E-01 3.07E-01 3.30E-01
G 31.7 3.19E-01 3.07E-01 3.16E-01
H 30.0 3.30E-01 3.07E-01

Protein purified on 3/30/2023 and assayed on 4/6/2023.
Data uploaded by VongphakdyK and last updated on 2023-04-13 10:55:45.


Citations

The unpublished data above were collected by students from Modesto Junior College. For inquiries, please contact Linda Brzezinski: <brzezinskil@yosemite.edu>.