Raw Data for BglB Variant H194N

Expression Data

SDS-PAGE
 0.85

Kinetic Assay Data

slope of H194N A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 4.00E-02 4.09E-02 3.67E-02
B 25.00 3.15E-02 3.46E-02 2.98E-02
C 8.33 2.67E-02 3.06E-02 2.39E-02
D 2.78 1.66E-02 1.78E-02 1.53E-02
E 0.93 6.65E-03 6.80E-03 5.15E-03
F 0.31 2.45E-03 2.55E-03 1.80E-03
G 0.10 1.15E-03 1.05E-03 7.50E-04
H 0.03 2.00E-04 3.50E-04 3.00E-04
 0.85      100×

Protein purified on 3/30/2023 and assayed on 3/31/2023.
Data uploaded by Natalie and last updated on 2023-04-13 10:19:36.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 8.01E-02 7.91E-02 7.97E-02
B 25.00 7.42E-02 7.12E-02 8.46E-02
C 8.33 5.76E-02 5.41E-02 5.63E-02
D 2.78 3.01E-02 3.81E-02 4.21E-02
E 0.93 1.40E-02 1.48E-02 1.46E-02
F 0.31 5.50E-03 5.55E-03 3.90E-03
G 0.10 1.95E-03 2.05E-03 2.05E-03
H 0.03 0 0 0
 1.59      100×

Protein purified on 3/30/2023 and assayed on 3/31/2023.
Data uploaded by dmg and last updated on 2023-04-13 10:26:05.


Thermostability Assay Data

slope of H194N A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 4.00E-04 0
B 48.8 0 0 5.00E-04
C 46.5 4.00E-04 4.00E-04 5.00E-04
D 42.5 9.10E-03 1.23E-02 1.66E-02
E 37.6 1.88E-01 2.00E-01 1.71E-01
F 33.8 2.09E-01 2.24E-01 2.50E-01
G 31.2 1.87E-01 1.92E-01 1.96E-01
H 30.0 1.83E-01 1.79E-01 2.04E-01

Protein purified on 3/30/2023 and assayed on 4/6/2023.
Data uploaded by Natalie and last updated on 2023-04-13 10:45:00.


Reference Data for WT

slope of H194N A420 nm (min−1)
T (°C) 1 2 3
A 50.0 4.00E-04 0 4.00E-04
B 48.8 4.00E-04 0 4.00E-04
C 46.5 1.10E-03 8.00E-04
D 42.5 9.54E-02 1.22E-01 6.22E-02
E 37.6 4.37E-01 4.30E-01 4.99E-01
F 33.8 4.18E-01 4.03E-01
G 31.2 2.41E-01 3.20E-01 3.05E-01
H 30.0 3.28E-01 3.13E-01 3.72E-01

Protein purified on 3/30/2023 and assayed on 4/6/2023.
Data uploaded by dmg and last updated on 2023-04-13 10:46:28.


Citations

The unpublished data above were collected by students from Modesto Junior College. For inquiries, please contact Linda Brzezinski: <brzezinskil@yosemite.edu>.