Raw Data for BglB Variant H194N

Expression Data

SDS-PAGE
 1.29

Kinetic Assay Data

slope of H194N A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 6.49E-02 6.66E-02 6.99E-02
B 25.00 5.79E-02 5.52E-02 4.72E-02
C 8.33 4.39E-02 4.35E-02 4.64E-02
D 2.78 2.66E-02 2.56E-02 2.53E-02
E 0.93 1.29E-02 1.15E-02 1.08E-02
F 0.31 4.45E-03 4.25E-03 4.50E-03
G 0.10 1.70E-03 1.55E-03
H 0.03 1.50E-04 2.00E-04 2.50E-04
 1.29      100×

Protein purified on 3/30/2023 and assayed on 3/31/2023.
Data uploaded by dmg and last updated on 2023-04-13 10:19:54.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 8.01E-02 7.91E-02 7.97E-02
B 25.00 7.42E-02 7.12E-02 8.46E-02
C 8.33 5.76E-02 5.41E-02 5.63E-02
D 2.78 3.01E-02 3.81E-02 4.21E-02
E 0.93 1.40E-02 1.48E-02 1.46E-02
F 0.31 5.50E-03 5.55E-03 3.90E-03
G 0.10 1.95E-03 2.05E-03 2.05E-03
H 0.03 0 0 0
 1.59      100×

Protein purified on 3/30/2023 and assayed on 3/31/2023.
Data uploaded by dmg and last updated on 2023-04-13 10:26:05.


Thermostability Assay Data

slope of H194N A420 nm (min−1)
T (°C) 1 2 3
A 50.0 4.00E-04 5.00E-04
B 48.8 1.20E-03 0 6.00E-04
C 46.5 5.00E-04 6.00E-04
D 42.5 1.95E-02 2.09E-02
E 37.6 1.61E-01 2.46E-01 2.83E-01
F 33.8 3.59E-01 3.54E-01 3.43E-01
G 31.2 3.11E-01 3.17E-01 3.52E-01
H 30.0 2.99E-01 3.20E-01 3.40E-01

Protein purified on 3/30/2023 and assayed on 4/6/2023.
Data uploaded by dmg and last updated on 2023-04-13 10:45:03.


Reference Data for WT

slope of H194N A420 nm (min−1)
T (°C) 1 2 3
A 50.0 4.00E-04 0 4.00E-04
B 48.8 4.00E-04 0 4.00E-04
C 46.5 1.10E-03 8.00E-04
D 42.5 9.54E-02 1.22E-01 6.22E-02
E 37.6 4.37E-01 4.30E-01 4.99E-01
F 33.8 4.18E-01 4.03E-01
G 31.2 2.41E-01 3.20E-01 3.05E-01
H 30.0 3.28E-01 3.13E-01 3.72E-01

Protein purified on 3/30/2023 and assayed on 4/6/2023.
Data uploaded by dmg and last updated on 2023-04-13 10:46:28.


Citations

The unpublished data above were collected by students from Modesto Junior College. For inquiries, please contact Linda Brzezinski: <brzezinskil@yosemite.edu>.