D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant K61F

Expression Data

Yield (mg/mL): 3.285

Kinetic Assay Data

**slope of K61F A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.13E-02	1.89E-02	1.91E-02
B	25.00	1.85E-02	1.82E-02	1.76E-02
C	8.33	1.34E-02	1.35E-02	1.61E-02
D	2.78	9.75E-03	8.85E-03	8.40E-03
E	0.93	4.35E-03	3.75E-03	3.75E-03
F	0.31	1.95E-03	1.95E-03	1.80E-03
G	0.10	1.05E-03	1.05E-03	9.00E-04
H	0.03	7.50E-04	7.50E-04	6.00E-04

Yield (mg/mL): 3.285 Dilution Factor: 100×

Protein purified on 3/24/23 and assayed on 3/31/23.
Data uploaded by murigs and last updated on 2023-04-14 06:48:23.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	7.31E-02	7.37E-02	7.28E-02
B	25.00	6.12E-02	6.89E-02	6.14E-02
C	8.33	5.87E-02	5.61E-02	5.45E-02
D	2.78	3.20E-02	3.08E-02	3.33E-02
E	0.93	1.40E-02	1.37E-02	1.46E-02
F	0.31	5.70E-03	5.40E-03	5.25E-03
G	0.10	2.25E-03	1.95E-03	2.70E-03
H	0.03	0	0	0

Yield (mg/mL): 1.683 Dilution Factor: 100×

Protein purified on 3/24/23 and assayed on 3/31/23.
Data uploaded by murigs and last updated on 2023-04-14 07:07:37.

Thermostability Assay Data

**slope of K61F A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	7.50E-04	6.00E-04
B	48.3	6.00E-04	6.00E-04	6.00E-04
C	45.7	6.00E-04	6.00E-04	7.50E-04
D	42.4	7.50E-04	6.00E-04	6.00E-04
E	37.7	1.50E-03	1.65E-03	1.50E-03
F	33.6	2.70E-03	3.45E-03	2.10E-03
G	31.3	3.45E-03	3.30E-03	2.85E-03
H	30.0	3.00E-03	3.00E-03	2.70E-03

Protein purified on 3/24/23 and assayed on 3/31/23.
Data uploaded by murigs and last updated on 2024-05-23 09:50:23.

Reference Data for WT

**slope of K61F A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	6.00E-04	6.00E-04	6.00E-04
B	48.3	0	9.00E-04	0
C	45.7	9.00E-04	7.50E-04	7.50E-04
D	42.4	4.95E-03	5.10E-03	4.65E-03
E	37.7	1.86E-02	1.79E-02	1.67E-02
F	33.6	2.52E-02	2.42E-02	2.31E-02
G	31.3	2.63E-02	2.66E-02	2.48E-02
H	30.0	2.57E-02	2.36E-02	1.92E-02

Protein purified on 3/24/2023 and assayed on 3/31/2023.
Data uploaded by pgarr022 and last updated on 2023-04-14 07:15:17.

Design Data

Rosetta Score for K61F: 7.625

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.