D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant K61W

Expression Data

Yield (mg/mL): 1.9395

Kinetic Assay Data

**slope of K61W A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.53E-02		4.13E-02
B	25.00	3.53E-02	3.57E-02	3.66E-02
C	8.33	2.88E-02	2.31E-02	2.70E-02
D	2.78	2.04E-02	1.92E-02	1.77E-02
E	0.93	9.45E-03	9.15E-03	9.00E-03
F	0.31	4.35E-03	3.75E-03	3.75E-03
G	0.10	2.10E-03	2.10E-03	1.80E-03
H	0.03	1.05E-03		7.50E-04

Yield (mg/mL): 1.9395 Dilution Factor: 100×

Protein purified on 3/24/23 and assayed on 3/31/23.
Data uploaded by mseig005 and last updated on 2023-04-14 07:38:18.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.27E-02	5.33E-02	5.34E-02
B	25.00	4.59E-02	4.59E-02	4.82E-02
C	8.33	3.86E-02	3.89E-02	3.86E-02
D	2.78	2.99E-02	2.72E-02	2.58E-02
E	0.93	1.17E-02	1.32E-02	1.20E-02
F	0.31	6.00E-03	6.00E-03	5.25E-03
G	0.10	2.70E-03	2.55E-03	2.55E-03
H	0.03	1.20E-03	0	7.50E-04

Yield (mg/mL): 1.683 Dilution Factor: 100×

Protein purified on 3/24/2023 and assayed on 3/31/2023.
Data uploaded by pgarr022 and last updated on 2023-04-14 06:54:26.

Thermostability Assay Data

**slope of K61W A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0.002400	1.05E-03	6.00E-04
B	48.3	6.00E-04		6.00E-04
C	45.7	7.50E-04
D	42.4	1.35E-03	1.35E-03	1.50E-03
E	37.7	6.15E-03	6.45E-03	6.90E-03
F	33.6	1.02E-02	9.60E-03	1.07E-02
G	31.3	1.05E-02		1.28E-02
H	30.0	7.80E-03	7.20E-03	7.50E-03

Protein purified on 3/24/23 and assayed on 3/31/23.
Data uploaded by mseig005 and last updated on 2023-04-14 07:35:49.

Reference Data for WT

**slope of K61W A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	6.00E-04	6.00E-04	6.00E-04
B	48.3	0	9.00E-04	0
C	45.7	9.00E-04	7.50E-04	7.50E-04
D	42.4	4.95E-03	5.10E-03	4.65E-03
E	37.7	1.86E-02	1.79E-02	1.67E-02
F	33.6	2.52E-02	2.42E-02	2.31E-02
G	31.3	2.63E-02	2.66E-02	2.48E-02
H	30.0	2.57E-02	2.36E-02	1.92E-02

Protein purified on 3/24/2023 and assayed on 3/31/2023.
Data uploaded by pgarr022 and last updated on 2023-04-14 07:15:17.

Design Data

Rosetta Score for K61W: 7.807

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.