Raw Data for BglB Variant K61F

Expression Data

SDS-PAGE
 1.866

Kinetic Assay Data

slope of K61F A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.06465 0.06375 0.07005
B 25.00 0.05805 0.0573 0.0618
C 8.33 0.0498 0.04215 0.0477
D 2.78 0.03285 0.0348 0.0348
E 0.93 0.0162 0.0162 0.01755
F 0.31 0.00675 0.0066 0.0072
G 0.10 0.00285 0.0033 0.0033
H 0.03 0 0
 1.866      100×

Protein purified on 2023-03-31 and assayed on 2023-03-31.
Data uploaded by ZieHenry and last updated on 2024-05-23 09:46:46.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 5.27E-02 5.33E-02 5.34E-02
B 25.00 4.59E-02 4.59E-02 4.82E-02
C 8.33 3.86E-02 3.89E-02 3.86E-02
D 2.78 2.99E-02 2.72E-02 2.58E-02
E 0.93 1.17E-02 1.32E-02 1.20E-02
F 0.31 6.00E-03 6.00E-03 5.25E-03
G 0.10 2.70E-03 2.55E-03 2.55E-03
H 0.03 1.20E-03 0 7.50E-04
 1.683      100×

Protein purified on 3/24/2023 and assayed on 3/31/2023.
Data uploaded by pgarr022 and last updated on 2023-04-14 06:54:26.


Thermostability Assay Data

slope of K61F A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0
B 48.3 0 0.00045
C 45.7 0.0009 0.0009
D 42.4 0.00225 0.00195 0.0018
E 37.7 0.0135 0.01335
F 33.6 0.0246 0.02955 0.02265
G 31.3 0.0282 0.02235
H 30.0 0.03375 0.02925 0.0369

Protein purified on 2023-03-24 and assayed on 2023-03-24.
Data uploaded by ZieHenry and last updated on 2024-05-23 09:47:25.


Reference Data for WT

slope of K61F A420 nm (min−1)
T (°C) 1 2 3
A 50.0 6.00E-04 6.00E-04 6.00E-04
B 48.3 0 9.00E-04 0
C 45.7 9.00E-04 7.50E-04 7.50E-04
D 42.4 4.95E-03 5.10E-03 4.65E-03
E 37.7 1.86E-02 1.79E-02 1.67E-02
F 33.6 2.52E-02 2.42E-02 2.31E-02
G 31.3 2.63E-02 2.66E-02 2.48E-02
H 30.0 2.57E-02 2.36E-02 1.92E-02

Protein purified on 3/24/2023 and assayed on 3/31/2023.
Data uploaded by pgarr022 and last updated on 2023-04-14 07:15:17.


Design Data

7.627

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.