Raw Data for BglB Variant K61N

Expression Data

SDS-PAGE
 3.285

Kinetic Assay Data

slope of K61N A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.1222 0.1203 0.1238
B 25.00 0.1017 0.09795 0.1055
C 8.33 0.09090 0.08520 0.09150
D 2.78 0.04695 0.05145 0.05415
E 0.93 0.02085 0.02055 0.01890
F 0.31 0.007800 0.008100 0.008700
G 0.10 0.002850 0.003000 0.003150
H 0.03 0 0.0004500 0.0007500
 3.285      100×

Protein purified on 3/24/2023 and assayed on 3/31/2023.
Data uploaded by cgome215 and last updated on 2023-04-14 07:08:20.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 7.31E-02 7.37E-02 7.28E-02
B 25.00 6.12E-02 6.89E-02 6.14E-02
C 8.33 5.87E-02 5.61E-02 5.45E-02
D 2.78 3.20E-02 3.08E-02 3.33E-02
E 0.93 1.40E-02 1.37E-02 1.46E-02
F 0.31 5.70E-03 5.40E-03 5.25E-03
G 0.10 2.25E-03 1.95E-03 2.70E-03
H 0.03 0 0 0
 1.683      100×

Protein purified on 3/24/23 and assayed on 3/31/23.
Data uploaded by murigs and last updated on 2023-04-14 07:07:37.


Thermostability Assay Data

slope of K61N A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0.0003000 0.0006000
B 48.3 0.0007500 0.0007500
C 45.7 0.0006002 0.0006000
D 42.4 0.002400 0.002400
E 37.7 0.01890 0.02040 0.01920
F 33.6 0.02985 0.02715 0.02745
G 31.3 0.03915 0.03510 0.02715
H 30.0 0.03300 0.03195

Protein purified on 3/24/2023 and assayed on 3/31/2023.
Data uploaded by cgome215 and last updated on 2024-05-23 09:41:36.


Reference Data for WT

slope of K61N A420 nm (min−1)
T (°C) 1 2 3
A 50.0 6.00E-04 6.00E-04 6.00E-04
B 48.3 0 9.00E-04 0
C 45.7 9.00E-04 7.50E-04 7.50E-04
D 42.4 4.95E-03 5.10E-03 4.65E-03
E 37.7 1.86E-02 1.79E-02 1.67E-02
F 33.6 2.52E-02 2.42E-02 2.31E-02
G 31.3 2.63E-02 2.66E-02 2.48E-02
H 30.0 2.57E-02 2.36E-02 1.92E-02

Protein purified on 3/24/2023 and assayed on 3/31/2023.
Data uploaded by pgarr022 and last updated on 2023-04-14 07:15:17.


Design Data

3.129

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.