Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.683

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	5.27E-02	5.33E-02	5.34E-02
	B	25.00	4.59E-02	4.59E-02	4.82E-02
	C	8.33	3.86E-02	3.89E-02	3.86E-02
	D	2.78	2.99E-02	2.72E-02	2.58E-02
	E	0.93	1.17E-02	1.32E-02	1.20E-02
	F	0.31	6.00E-03	6.00E-03	5.25E-03
	G	0.10	2.70E-03	2.55E-03	2.55E-03
	H	0.03	1.20E-03	0	7.50E-04

Yield (mg/mL): 1.683     Dilution Factor:  100×

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Protein purified on 3/24/2023 and assayed on 3/31/2023.
Data uploaded by pgarr022 and last updated on 2023-04-14 06:54:26.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	6.00E-04	6.00E-04	6.00E-04
	B	48.3	0	9.00E-04	0
	C	45.7	9.00E-04	7.50E-04	7.50E-04
	D	42.4	4.95E-03	5.10E-03	4.65E-03
	E	37.7	1.86E-02	1.79E-02	1.67E-02
	F	33.6	2.52E-02	2.42E-02	2.31E-02
	G	31.3	2.63E-02	2.66E-02	2.48E-02
	H	30.0	2.57E-02	2.36E-02	1.92E-02

Download these data

Protein purified on 3/24/2023 and assayed on 3/31/2023.
Data uploaded by pgarr022 and last updated on 2023-04-14 07:15:17.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.