D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant K61L

Expression Data

Yield (mg/mL): 0.82545

Kinetic Assay Data

**slope of K61L A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.01650		0.01545
B	25.00	0.01320	0.01320	0.01305
C	8.33	0.01035	0.01020	0.01035
D	2.78	0.009450	0.007950	0.008100
E	0.93	0.004800	0.004050	0.004500
F	0.31	0.001950	0.001950	0.002100
G	0.10	0.001500	0.001050	0.001200
H	0.03		0.0006000	0.0007500

Yield (mg/mL): 0.82545 Dilution Factor: 100×

Protein purified on 3/24/23 and assayed on 3/31/23.
Data uploaded by Sebastian and last updated on 2023-04-14 07:39:16.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.27E-02	5.33E-02	5.34E-02
B	25.00	4.59E-02	4.59E-02	4.82E-02
C	8.33	3.86E-02	3.89E-02	3.86E-02
D	2.78	2.99E-02	2.72E-02	2.58E-02
E	0.93	1.17E-02	1.32E-02	1.20E-02
F	0.31	6.00E-03	6.00E-03	5.25E-03
G	0.10	2.70E-03	2.55E-03	2.55E-03
H	0.03	1.20E-03	0	7.50E-04

Yield (mg/mL): 1.683 Dilution Factor: 100×

Protein purified on 3/24/2023 and assayed on 3/31/2023.
Data uploaded by pgarr022 and last updated on 2023-04-14 06:54:26.

Thermostability Assay Data

**slope of K61L A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0		0.0004500	0.0007500
B	48.3	0.0007500	0.0006000	0.0007500
C	45.7	0.002100	0.0009000
D	42.4	0.001050	0.001050
E	37.7	0.002100	0.001950	0.001800
F	33.6	0.003300	0.002850	0.003600
G	31.3	0.003750	0.003600	0.003750
H	30.0	0.003301	0.003450	0.003300

Protein purified on 3/24/23 and assayed on 3/31/23.
Data uploaded by Sebastian and last updated on 2023-04-14 07:32:52.

Reference Data for WT

**slope of K61L A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	6.00E-04	6.00E-04	6.00E-04
B	48.3	0	9.00E-04	0
C	45.7	9.00E-04	7.50E-04	7.50E-04
D	42.4	4.95E-03	5.10E-03	4.65E-03
E	37.7	1.86E-02	1.79E-02	1.67E-02
F	33.6	2.52E-02	2.42E-02	2.31E-02
G	31.3	2.63E-02	2.66E-02	2.48E-02
H	30.0	2.57E-02	2.36E-02	1.92E-02

Protein purified on 3/24/2023 and assayed on 3/31/2023.
Data uploaded by pgarr022 and last updated on 2023-04-14 07:15:17.

Design Data

Rosetta Score for K61L: 5.534

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.