D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant K61A

Expression Data

Yield (mg/mL): 1.549

Kinetic Assay Data

**slope of K61A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	7.02E-02	7.65E-02	8.06E-02
B	25.00	6.26E-02	6.11E-02	6.23E-02
C	8.33	5.34E-02	5.06E-02	6.42E-02
D	2.78	3.23E-02	3.65E-02	2.99E-02
E	0.93	1.28E-02	1.41E-02	1.34E-02
F	0.31	5.40E-03	5.55E-03	5.10E-03
G	0.10	2.10E-03	2.25E-03	2.10E-03
H	0.03	0.00E+00	0.00E+00	0.00E+00

Yield (mg/mL): 1.549 Dilution Factor: 100×

Protein purified on 3/24/23 and assayed on 3/31/23.
Data uploaded by cmart638 and last updated on 2023-04-20 12:32:29.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	7.31E-02	7.37E-02	7.28E-02
B	25.00	6.12E-02	6.89E-02	6.14E-02
C	8.33	5.87E-02	5.61E-02	5.45E-02
D	2.78	3.20E-02	3.08E-02	3.33E-02
E	0.93	1.40E-02	1.37E-02	1.46E-02
F	0.31	5.70E-03	5.40E-03	5.25E-03
G	0.10	2.25E-03	1.95E-03	2.70E-03
H	0.03	0	0	0

Yield (mg/mL): 1.683 Dilution Factor: 100×

Protein purified on 3/24/23 and assayed on 3/31/23.
Data uploaded by murigs and last updated on 2023-04-14 07:07:37.

Thermostability Assay Data

**slope of K61A A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	6.00E-04	6.00E-04
B	48.3	7.50E-04		6.00E-04
C	45.7	9.00E-04	6.00E-04	1.05E-03
D	42.4	3.00E-03	3.15E-03	3.15E-03
E	37.7	1.98E-02	1.89E-02	1.92E-02
F	33.6	2.43E-02	2.57E-02	2.54E-02
G	31.3	3.29E-02	2.87E-02
H	30.0	2.61E-02	2.40E-02	2.15E-02

Protein purified on 3/24/23 and assayed on 3/31/23.
Data uploaded by cmart638 and last updated on 2024-05-23 09:31:33.

Reference Data for WT

**slope of K61A A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	6.00E-04	4.50E-04	9.00E-04
B	48.3
C	45.7	9.00E-04	9.00E-04	7.50E-04
D	42.4	3.75E-03	3.75E-03	3.45E-03
E	37.7	1.64E-02	1.73E-02	1.65E-02
F	33.6	2.19E-02	2.33E-02	2.19E-02
G	31.3	2.33E-02	2.28E-02	2.16E-02
H	30.0	2.04E-02	2.19E-02

Protein purified on 3/24/2023 and assayed on 3/31/2023.
Data uploaded by murigs and last updated on 2024-05-23 09:39:36.

Design Data

Rosetta Score for K61A: 1.347

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.