Raw Data for BglB Variant K61I

Expression Data

SDS-PAGE
 1.7975

Kinetic Assay Data

slope of K61I A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.0549 0.0588
B 25.00 0.0495 0.04935 0.05055
C 8.33 0.04275 0.03975 0.0396
D 2.78 0.0321 0.03045 0.03435
E 0.93 0.01515 0.0144 0.01365
F 0.31 0.00675 0.006 0.00705
G 0.10 0.0024 0.0024 0.00315
H 0.03 0 0
 1.7975      100×

Protein purified on 3/24/2023 and assayed on 3/31/2023.
Data uploaded by cdang011 and last updated on 2024-05-23 09:29:34.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 5.27E-02 5.33E-02 5.34E-02
B 25.00 4.59E-02 4.59E-02 4.82E-02
C 8.33 3.86E-02 3.89E-02 3.86E-02
D 2.78 2.99E-02 2.72E-02 2.58E-02
E 0.93 1.17E-02 1.32E-02 1.20E-02
F 0.31 6.00E-03 6.00E-03 5.25E-03
G 0.10 2.70E-03 2.55E-03 2.55E-03
H 0.03 1.20E-03 0 7.50E-04
 1.683      100×

Protein purified on 3/24/2023 and assayed on 3/31/2023.
Data uploaded by pgarr022 and last updated on 2023-04-14 06:54:26.


Thermostability Assay Data

slope of K61I A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00075 0.00075
B 48.3 0 0
C 45.7 0.00075 0.0007502
D 42.4 0.00315 0.00285 0.003
E 37.7 0.0171 0.01575 0.0174
F 33.6 0.02715 0.0243 0.02535
G 31.3 0.02955 0.0273 0.0252
H 30.0 0.027 0.0234

Protein purified on 3/24/2023 and assayed on 3/31/2023.
Data uploaded by cdang011 and last updated on 2024-05-23 09:30:09.


Reference Data for WT

slope of K61I A420 nm (min−1)
T (°C) 1 2 3
A 50.0 6.00E-04 6.00E-04 6.00E-04
B 48.3 0 9.00E-04 0
C 45.7 9.00E-04 7.50E-04 7.50E-04
D 42.4 4.95E-03 5.10E-03 4.65E-03
E 37.7 1.86E-02 1.79E-02 1.67E-02
F 33.6 2.52E-02 2.42E-02 2.31E-02
G 31.3 2.63E-02 2.66E-02 2.48E-02
H 30.0 2.57E-02 2.36E-02 1.92E-02

Protein purified on 3/24/2023 and assayed on 3/31/2023.
Data uploaded by pgarr022 and last updated on 2023-04-14 07:15:17.


Design Data

1.12

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.