Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.159

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	8.21E-02	5.46E-02
	B	25.00		6.29E-02	5.49E-02
	C	8.33	5.96E-02	4.01E-02	5.10E-02
	D	2.78		2.87E-02	2.43E-02
	E	0.93	9.60E-03	1.40E-02	1.68E-02
	F	0.31		5.85E-03	5.70E-03
	G	0.10	2.40E-03	2.85E-03	2.85E-03
	H	0.03	6.00E-04	7.50E-04	0

Yield (mg/mL): 1.159     Dilution Factor:  100×

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Protein purified on 3/24/23 and assayed on 3/31/23.
Data uploaded by sabrinamansoor and last updated on 2023-04-18 09:38:49.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0	0	4.50E-04
	B	48.3	7.50E-04
	C	45.7	6.00E-04	7.50E-04	7.50E-04
	D	42.4	1.20E-02	1.34E-02	1.22E-02
	E	37.7	3.12E-01	2.94E-01	2.77E-01
	F	33.6	2.60E-01	2.72E-01	3.01E-01
	G	31.3	3.38E-01	3.54E-01	3.54E-01
	H	30.0	3.17E-01	3.00E-01	3.08E-01

Download these data

Protein purified on 3/24/23 and assayed on 3/31/23.
Data uploaded by sabrinamansoor and last updated on 2023-04-18 09:38:20.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.