D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant A306S

Expression Data

Yield (mg/mL): 0.625

Kinetic Assay Data

**slope of A306S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	7.25E-02	5.98E-02	6.93E-02
B	25.00	5.21E-02	4.72E-02	4.87E-02
C	8.33	3.35E-02	3.00E-02	2.82E-02
D	2.78	1.82E-02	1.77E-02	1.71E-02
E	0.93	9.34E-03	8.24E-03	8.37E-03
F	0.31	3.89E-03	3.50E-03	3.42E-03
G	0.10	1.32E-03	1.69E-03	1.46E-03
H	0.03	2.17E-04	1.39E-04	0

Yield (mg/mL): 0.625 Dilution Factor: 100×

Protein purified on 4/13/2023 and assayed on 4/14/2023.
Data uploaded by kasiapno and last updated on 2023-04-17 15:23:29.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	8.90E-02	8.91E-02	7.56E-02
B	25.00	7.95E-02	7.43E-02	7.78E-02
C	8.33	6.00E-02	5.31E-02	5.09E-02
D	2.78	2.99E-02	2.85E-02	2.73E-02
E	0.93	1.42E-02	1.44E-02	1.25E-02
F	0.31	5.18E-03	6.47E-03	5.69E-03
G	0.10	1.81E-03	2.01E-03	2.05E-03
H	0.03	1.32E-04	1.19E-04

Yield (mg/mL): 0.777 Dilution Factor: 100×

Protein purified on 4/13/2023 and assayed on 4/14/2023.
Data uploaded by kasiapno and last updated on 2023-04-17 16:25:44.

Design Data

Rosetta Score for A306S: -1.688

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.