D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N293A

Expression Data

Yield (mg/mL): 0.791

Kinetic Assay Data

**slope of N293A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.60E-02	1.72E-02	1.44E-02
B	25.00	1.16E-02	1.19E-02	1.07E-02
C	8.33	7.17E-03	6.70E-03	6.62E-03
D	2.78	4.33E-03	4.03E-03	3.11E-03
E	0.93	1.14E-03	1.13E-03	1.22E-03
F	0.31	5.77E-04	4.77E-04	3.67E-04
G	0.10	3.39E-04		3.73E-04
H	0.03	3.47E-04	2.63E-04	1.79E-04

Yield (mg/mL): 0.791 Dilution Factor: 10×

Protein purified on 4/13/2023 and assayed on 4/14/2023.
Data uploaded by kasiapno and last updated on 2023-04-17 16:40:28.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	8.90E-02	8.91E-02	7.56E-02
B	25.00	7.95E-02	7.43E-02	7.78E-02
C	8.33	6.00E-02	5.31E-02	5.09E-02
D	2.78	2.99E-02	2.85E-02	2.73E-02
E	0.93	1.42E-02	1.44E-02	1.25E-02
F	0.31	5.18E-03	6.47E-03	5.69E-03
G	0.10	1.81E-03	2.01E-03	2.05E-03
H	0.03	1.32E-04	1.19E-04

Yield (mg/mL): 0.777 Dilution Factor: 100×

Protein purified on 4/13/2023 and assayed on 4/14/2023.
Data uploaded by kasiapno and last updated on 2023-04-17 16:25:44.

Design Data

Rosetta Score for N293A: -0.413

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.