Raw Data for BglB Variant Q124I

Expression Data

SDS-PAGE
 1.74

Kinetic Assay Data

slope of Q124I A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 9.57E-02 1.01E-01 9.43E-02
B 25.00 8.44E-02 7.88E-02 8.45E-02
C 8.33 5.73E-02 6.12E-02 6.00E-02
D 2.78 3.52E-02 4.39E-02 3.73E-02
E 0.93 1.72E-02 2.07E-02 1.61E-02
F 0.31 6.75E-03 6.95E-03
G 0.10 2.45E-03 3.85E-03 2.85E-03
H 0.03 0 0 0
 1.74      10×

Protein purified on 3/27/2023 and assayed on 3/31/2023.
Data uploaded by Jesse and last updated on 2023-04-17 17:04:44.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 7.15E-02 7.22E-02 7.17E-02
B 25.00 5.55E-02 6.19E-02 5.91E-02
C 8.33 4.44E-02 4.47E-02 4.70E-02
D 2.78 3.04E-02 2.80E-02 3.42E-02
E 0.93 1.72E-02 1.31E-02 1.78E-02
F 0.31 6.95E-03 5.20E-03 7.90E-03
G 0.10 2.50E-03 2.10E-03 2.75E-03
H 0.03 0 2.00E-04 0
 2.41      100×

Protein purified on 3/27/2023 and assayed on 3/31/2023.
Data uploaded by jayden921458 and last updated on 2023-04-17 17:02:00.


Thermostability Assay Data

slope of Q124I A420 nm (min−1)
T (°C) 1 2 3
A 50.0 8.00E-04 7.00E-04 8.00E-04
B 48.8 1.00E-03 9.00E-04
C 46.5 1.30E-03 1.20E-03 9.00E-04
D 42.5 1.05E-01 6.12E-02 7.77E-02
E 37.6 2.64E-01 2.68E-01 2.47E-01
F 33.8 2.81E-01 2.99E-01 3.01E-01
G 31.2 2.84E-01 2.88E-01 2.99E-01
H 30.0 3.05E-01 3.08E-01 3.25E-01

Protein purified on 3/27/2023 and assayed on 4/3/2023.
Data uploaded by Jesse and last updated on 2023-04-17 17:17:44.


Reference Data for WT

slope of Q124I A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.001500 0.001000 0
B 48.8 0 0.0005000 0.0008000
C 46.5 0.001200 0.001000
D 42.5 0.03320 0.02810 0.02460
E 37.6 0.08170 0.06340 0.07460
F 33.8 0.09360 0.08560 0.07760
G 31.2 0.08079 0.08150
H 30.0 0.1009 0.08870 0.09760

Protein purified on 3/27/2023 and assayed on 4/3/2023.
Data uploaded by jayden921458 and last updated on 2023-04-17 17:31:06.


Citations

The unpublished data above were collected by students from Modesto Junior College. For inquiries, please contact Linda Brzezinski: <brzezinskil@yosemite.edu>.