D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant Q124S

Expression Data

Yield (mg/mL): 0.61

Kinetic Assay Data

**slope of Q124S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.23E-02	2.67E-02	2.10E-02
B	25.00	1.84E-02	2.36E-02	2.14E-02
C	8.33	1.56E-02	1.84E-02	1.42E-02
D	2.78	6.80E-03	7.75E-03	6.65E-03
E	0.93	4.15E-03	3.85E-03	4.05E-03
F	0.31	1.55E-03	1.75E-03	1.70E-03
G	0.10	7.50E-04	7.00E-04	7.50E-04
H	0.03	0	2.00E-04	2.00E-04

Yield (mg/mL): 0.61 Dilution Factor: 100×

Protein purified on 3/27/2023 and assayed on 3/31/2023.
Data uploaded by Yasmin and last updated on 2023-04-17 16:54:40.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		3.43E-02	3.41E-02
B	25.00	3.38E-02	3.30E-02	3.37E-02
C	8.33	2.62E-02	2.68E-02	2.51E-02
D	2.78	1.53E-02	1.58E-02	1.58E-02
E	0.93	7.00E-03	6.75E-03	7.20E-03
F	0.31	2.65E-03	2.60E-03	2.80E-03
G	0.10	1.10E-03	1.10E-03	1.10E-03
H	0.03	4.00E-04	0	2.50E-04

Yield (mg/mL): 0.72 Dilution Factor: 100×

Protein purified on 3/27/2023 and assayed on 3/31/2023.
Data uploaded by Niconicolas and last updated on 2023-04-17 16:49:50.

Thermostability Assay Data

Data missing or deleted from the database.

Reference Data for WT

**slope of Q124S A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0		5.00E-04	5.00E-04
B	48.8	3.00E-04		4.00E-04
C	46.5	1.10E-03	1.00E-03	9.00E-04
D	42.5		5.14E-02	4.76E-02
E	37.6	2.13E-01	1.84E-01	1.86E-01
F	33.8	1.93E-01	1.97E-01	1.98E-01
G	31.2		1.99E-01	2.05E-01
H	30.0	1.91E-01	2.05E-01	1.96E-01

Protein purified on 3/27/2023 and assayed on 4/3/2023.
Data uploaded by Niconicolas and last updated on 2023-04-17 17:13:35.

Citations

The unpublished data above were collected by students from Modesto Junior College. For inquiries, please contact Linda Brzezinski: <brzezinskil@yosemite.edu>.