D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant Q124M

Expression Data

Yield (mg/mL): 0.875

Kinetic Assay Data

**slope of Q124M A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.67E-01	4.57E-01	4.76E-01
B	25.00	3.82E-01	4.03E-01	4.03E-01
C	8.33	2.89E-01	2.98E-01	2.81E-01
D	2.78	1.80E-01	1.71E-01	1.99E-01
E	0.93	7.84E-02	7.67E-02	7.43E-02
F	0.31	3.09E-02	2.69E-02	3.20E-02
G	0.10	9.40E-03	8.55E-03	9.05E-03
H	0.03	1.00E-04	2.00E-04	0

Yield (mg/mL): 0.875 Dilution Factor: 10×

Protein purified on 3/27/2023 and assayed on 3/31/2023.
Data uploaded by nsihdu24 and last updated on 2023-04-17 16:56:21.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.18E-02	5.52E-02	5.54E-02
B	25.00	4.95E-02	5.28E-02	5.38E-02
C	8.33	3.67E-02	3.83E-02	3.82E-02
D	2.78	2.11E-02	2.67E-02	2.21E-02
E	0.93	8.50E-03	1.05E-02	1.05E-02
F	0.31	3.35E-03		4.25E-03
G	0.10	1.30E-03		1.45E-03
H	0.03	2.00E-04	0	2.50E-04

Yield (mg/mL): 1.556 Dilution Factor: 100×

Protein purified on 3/27/2023 and assayed on 3/31/2023.
Data uploaded by Mahnoor and last updated on 2023-04-17 17:00:45.

Thermostability Assay Data

**slope of Q124M A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	1.00E-03	0	4.00E-04
B	48.8	4.00E-04	0	4.00E-04
C	46.5	4.00E-04	4.00E-04	0
D	42.5	2.02E-01	1.97E-01	1.75E-01
E	37.6	1.99E-01		2.01E-01
F	33.8	2.53E-01	2.16E-01	2.83E-01
G	31.2	2.72E-01	2.19E-01	2.35E-01
H	50.0	2.74E-01	2.86E-01	2.92E-01

Protein purified on 3/27/2023 and assayed on 4/3/2023.
Data uploaded by nsihdu24 and last updated on 2023-04-17 17:22:04.

Reference Data for WT

**slope of Q124M A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0	5.00E-04	5.00E-04
B	48.8	6.00E-04	0	4.00E-04
C	46.5	1.00E-03	1.20E-03	7.00E-04
D	42.5	9.85E-02	9.03E-02	9.18E-02
E	37.6	6.03E-02		6.48E-02
F	33.8	9.21E-02	1.00E-01	8.46E-02
G	31.2	9.85E-02	8.43E-02	1.01E-01
H	30.0	9.26E-02	9.86E-02	1.01E-01

Protein purified on 3/27/2023 and assayed on 4/3/2023.
Data uploaded by Mahnoor and last updated on 2023-04-17 17:18:48.

Citations

The unpublished data above were collected by students from Modesto Junior College. For inquiries, please contact Linda Brzezinski: <brzezinskil@yosemite.edu>.