D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant I91A

Yield (mg/mL): 0.107

**slope of I91A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.44E-01	1.44E-01
B	25.00	1.16E-01	1.13E-01	1.10E-01
C	8.33	8.76E-02	9.25E-02	8.69E-02
D	2.78	5.49E-02	5.41E-02	5.73E-02
E	0.93	2.11E-02	2.10E-02	2.53E-02
F	0.31	7.90E-03	7.85E-03	8.15E-03
G	0.10	3.15E-03	2.70E-03	3.15E-03
H	0.03	0.00E+00	0.00E+00	0.00E+00

Yield / A_{280 nm}: 0.107 Dilution Factor: 10×

Protein purified on 4/27/23 and assayed on 5/6/23.
Data uploaded by LeivBerg and last updated on 2023-05-18 11:23:07.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.06E-02	4.93E-02	4.98E-02
B	25.00	3.93E-02	3.87E-02	3.85E-02
C	8.33	3.19E-02	3.02E-02	3.21E-02
D	2.78	2.04E-02	1.82E-02	1.70E-02
E	0.93	6.65E-03	6.75E-03	7.00E-03
F	0.31	2.90E-03	2.55E-03	2.70E-03
G	0.10	1.00E-03	1.25E-03	1.05E-03
H	0.03	2.50E-04	1.50E-04	0.00E+00

Yield / A_{280 nm}: 0.177 Dilution Factor: 10×

Protein purified on 4/27/2023 and assayed on 5/6/2023.
Data uploaded by ToniB and last updated on 2023-05-18 11:26:43.

**slope of I91A A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0	0	0
B	48.3	2.50E-04	2.50E-04
C	45.7	4.00E-04	4.00E-04
D	42.4	5.05E-03	5.30E-03	5.55E-03
E	37.7	6.35E-02	6.35E-02	6.52E-02
F	33.6	7.56E-02	7.67E-02	7.47E-02
G	31.3	7.78E-02	7.79E-02	7.63E-02
H	30.0	7.92E-02	7.80E-02	7.83E-02

Protein purified on 4/27/23 and assayed on 5/8/23.
Data uploaded by reynardlacson and last updated on 2023-05-18 11:23:08.

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Rosetta Score for I91A: -2.767

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.