D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant I91R

Yield (mg/mL): 0.156

**slope of I91R A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.44E-02	5.38E-02	5.45E-02
B	25.00	4.35E-02	4.37E-02	4.32E-02
C	8.33	3.11E-02	3.30E-02	3.19E-02
D	2.78	1.70E-02	1.77E-02	2.07E-02
E	0.93	7.95E-03	8.50E-03	7.95E-03
F	0.31		3.25E-03	3.20E-03
G	0.10	1.40E-03	1.25E-03	1.25E-03
H	0.03		2.00E-04	2.00E-04

Yield / A_{280 nm}: 0.156 Dilution Factor: 10×

Protein purified on 4/27/23 and assayed on 5/6/23.
Data uploaded by Katrisse and last updated on 2023-05-18 11:26:12.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00	2.91E-02	2.85E-02	2.88E-02
C	8.33	2.31E-02	2.25E-02	2.32E-02
D	2.78	1.41E-02	1.39E-02	1.40E-02
E	0.93	6.55E-03	6.35E-03	6.30E-03
F	0.31	2.60E-03	2.40E-03	2.40E-03
G	0.10	1.05E-03	1.00E-03	9.50E-04
H	0.03	2.00E-04	1.50E-04	2.50E-04

Yield (mg/mL): 0.310937 Dilution Factor: 100×

Protein purified on 11/9/2022 and assayed on 11/16/2022.
Data uploaded by DanielleBeverSneary and last updated on 2022-12-12 09:19:22.

**slope of I91R A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0	0
B	48.3	2.00E-04	2.50E-04
C	45.7	2.50E-04	2.50E-04	2.50E-04
D	42.4	2.10E-03	1.85E-03	1.75E-03
E	37.7	2.02E-02	1.96E-02	1.92E-02
F	33.6	2.50E-02	2.64E-02	2.45E-02
G	31.3	2.76E-02	2.51E-02	2.49E-02
H	30.0	2.61E-02	2.32E-02	2.27E-02

Protein purified on 4/27/23 and assayed on 5/8/23.
Data uploaded by Katrisse and last updated on 2024-12-20 20:50:01.

**slope of I91R A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	3.00E-04	2.50E-04	1.50E-04
B	48.3	2.50E-04	2.50E-04
C	45.7	1.10E-03	7.50E-04	5.50E-04
D	42.4	9.00E-03	8.00E-03	8.95E-03
E	37.7	2.72E-02	2.77E-02	2.71E-02
F	33.6	3.15E-02	3.26E-02	2.93E-02
G	31.3	3.38E-02	3.48E-02	3.22E-02
H	30.0	3.52E-02	3.53E-02	3.36E-02

Protein purified on 4/27/2023 and assayed on 5/8/2023.
Data uploaded by tolsy2000 and last updated on 2023-05-18 11:38:46.

Rosetta Score for I91R: -8.028

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.