Raw Data for BglB Variant I91A

Expression Data

Yield (mg/mL): 147

Kinetic Assay Data

slope of I91A A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	5.83E-02	6.01E-02	6.14E-02
	B	25.00	4.62E-02	4.78E-02	4.94E-02
	C	8.33	3.24E-02	3.31E-02	3.75E-02
	D	2.78	1.97E-02	2.21E-02	2.17E-02
	E	0.93	9.20E-03	9.30E-03	9.95E-03
	F	0.31	3.25E-03	3.40E-03	4.30E-03
	G	0.10	1.35E-03	1.30E-03	1.30E-03
	H	0.03	4.30E-03	2.50E-04	2.50E-03

Yield / A_{280 nm}: 147     Dilution Factor:  10×

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Protein purified on 4/27/23 and assayed on 5/4/23.
Data uploaded by Karla and last updated on 2023-06-01 10:22:27.

Reference Data for WT

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	5.06E-02	4.93E-02	4.98E-02
	B	25.00	3.93E-02	3.87E-02	3.85E-02
	C	8.33	3.19E-02	3.02E-02	3.21E-02
	D	2.78	2.04E-02	1.82E-02	1.70E-02
	E	0.93	6.65E-03	6.75E-03	7.00E-03
	F	0.31	2.90E-03	2.55E-03	2.70E-03
	G	0.10	1.00E-03	1.25E-03	1.05E-03
	H	0.03	2.50E-04	1.50E-04	0.00E+00

Yield / A_{280 nm}: 0.177     Dilution Factor:  10×

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Protein purified on 4/27/2023 and assayed on 5/6/2023.
Data uploaded by ToniB and last updated on 2023-05-18 11:26:43.

Thermostability Assay Data

slope of I91A A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.0002500	0.0003000	0.0002000
	B	48.3	0		0
	C	45.7		0.0002500	0.0003000
	D	42.4	0.001250	0.001550	0.001650
	E	37.7	0.02340	0.02265	0.02285
	F	33.6	0.02950	0.02940	0.02960
	G	31.3	0.02975	0.02905	0.02935
	H	30.0	0.02905	0.02930	0.03015

Download these data

Protein purified on 4/27/2023 and assayed on 5/8/2023.
Data uploaded by jadechen and last updated on 2023-05-18 11:20:51.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Design Data

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.