Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.291

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	7.38E-02	7.57E-02	7.24E-02
	B	25.00	6.23E-02	6.03E-02	5.61E-02
	C	8.33	4.70E-02	4.69E-02	4.26E-02
	D	2.78	2.55E-02	2.71E-02	2.58E-02
	E	0.93	1.22E-02	1.13E-02	1.16E-02
	F	0.31	4.40E-03	4.30E-03	4.60E-03
	G	0.10	1.70E-03		1.60E-03
	H	0.03	0.00E+00	0.00E+00

Yield (mg/mL): 0.291     Dilution Factor:  10×

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Protein purified on 2/7/23 and assayed on 5/6/23.
Data uploaded by delaneycarpenter and last updated on 2023-05-18 11:14:16.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	3.00E-04	2.50E-04	1.50E-04
	B	48.3	2.50E-04	2.50E-04
	C	45.7	1.10E-03	7.50E-04	5.50E-04
	D	42.4	9.00E-03	8.00E-03	8.95E-03
	E	37.7	2.72E-02	2.77E-02	2.71E-02
	F	33.6	3.15E-02	3.26E-02	2.93E-02
	G	31.3	3.38E-02	3.48E-02	3.22E-02
	H	30.0	3.52E-02	3.53E-02	3.36E-02

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Protein purified on 4/27/2023 and assayed on 5/8/2023.
Data uploaded by tolsy2000 and last updated on 2023-05-18 11:38:46.

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.