Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.6

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	2.51E-01	2.43E-01	2.50E-01
	B	25.00	1.97E-01	1.81E-01	1.88E-01
	C	8.33
	D	2.78
	E	0.93	4.21E-02	4.13E-02	4.13E-02
	F	0.31	1.32E-02	1.10E-02	1.06E-02
	G	0.10	3.05E-03		2.80E-03
	H	0.03		2.60E-03	2.40E-03

Yield (mg/mL): 1.6     Dilution Factor:  100×

Download these data

Protein purified on 5/17/23 and assayed on 5/24/23.
Data uploaded by eLi and last updated on 2023-05-31 10:28:58.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	6.00E-04	0	4.00E-04
	B	48.3	0	0
	C	45.7	1.00E-03	1.00E-03	9.00E-04
	D	42.4	1.35E-02	1.26E-02	1.15E-02
	E	37.7	5.94E-02	5.81E-02	5.47E-02
	F	33.6		6.24E-02	5.23E-02
	G	31.3	5.97E-02	6.21E-02	6.54E-02
	H	30.0	5.93E-02	6.38E-02	6.48E-02

Download these data

Protein purified on 5/17/23 and assayed on 5/24/23.
Data uploaded by eLi and last updated on 2023-05-31 10:37:46.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.