Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.5

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	2.57E-01	2.34E-01	2.37E-01
	B	25.00	1.91E-01	1.95E-01	1.88E-01
	C	8.33	1.58E-01	1.67E-01	1.84E-01
	D	2.78	9.05E-02	9.59E-02	9.26E-02
	E	0.93	3.90E-02	4.11E-02	4.26E-02
	F	0.31	1.50E-02	1.47E-02	1.35E-02
	G	0.10	6.65E-03	9.80E-03	4.75E-03
	H	0.03	2.15E-03		2.45E-03

Yield (mg/mL): 0.5     Dilution Factor:  100×

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Protein purified on 5/17/23 and assayed on 5/24/23.
Data uploaded by NicoleH and last updated on 2023-05-31 11:02:52.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	1.02E-03		3.99E-04
	B	48.3	1.02E-03		3.99E-04
	C	45.7	3.51E-04	3.21E-04	3.68E-04
	D	42.4	2.69E-02	2.89E-02	3.51E-02
	E	37.7	2.07E-01	2.07E-01	2.07E-01
	F	33.6	2.00E-01	1.75E-01	1.93E-01
	G	31.3	2.05E-01	2.03E-01	2.05E-01
	H	30.0	2.07E-01	1.75E-01	2.02E-01

Download these data

Protein purified on 5/17/23 and assayed on 5/24/23.
Data uploaded by NicoleH and last updated on 2023-05-31 11:07:10.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.