D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant I170Y

Expression Data

Yield (mg/mL): 0.860081

Kinetic Assay Data

**slope of I170Y A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.10841	0.11297	0.10862
B	25.00	0.082655	0.091345	0.082345
C	8.33	0.064552	0.06931	0.067862
D	2.78	0.047276	0.042931	0.041586
E	0.93	0.019448	0.020379	0.019138
F	0.31	0.0083793	0.0086897	0.0078621
G	0.10	0.0046552	0.0051724	0.0051724
H	0.03	0.0027931		0.0021724

Yield (mg/mL): 0.860081 Dilution Factor: 100×

Protein purified on 7/17/23 and assayed on 7/18/23.
Data uploaded by annikabennett and last updated on 2023-07-20 11:58:07.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.05E-01	3.26E-01	3.35E-01
B	25.00	2.62E-01	2.82E-01	3.00E-01
C	8.33	2.08E-01	2.31E-01	2.11E-01
D	2.78	1.22E-01	1.32E-01	1.23E-01
E	0.93	5.61E-02	5.47E-02	6.32E-02
F	0.31	2.19E-02	2.15E-02	2.18E-02
G	0.10	8.69E-03	8.07E-03	9.21E-03
H	0.03	0		0

Yield (mg/mL): 1.63825 Dilution Factor: 100×

Protein purified on 7/17/2023 and assayed on 7/18/2023.
Data uploaded by carterahlstedt and last updated on 2023-07-20 11:00:12.

Thermostability Assay Data

**slope of I170Y A_{420 nm} (min⁻¹)**
	T (°C)	1	2
A	30.0	0.15466	0.16324
B	30.5	0.12786	0.12972
C	31.7	0.12972	0.12683
D	33.2	0.099	0.09931
E	35.4	0.059793	0.060621
F	38.3	0.016966	0.052345
G	41.7	0.0031034	0.0040345
H	44.5	0.0038276	0
I	46.8	0	0
J	48.3	0	0
K	49.5	0.0015517	0
L	50.0	0.0084828	0

Protein purified on 7/17/23 and assayed on 7/19/23.
Data uploaded by annikabennett and last updated on 2023-07-20 12:17:53.

Reference Data for WT

**slope of I170Y A_{420 nm} (min⁻¹)**
	T (°C)	1	2
A	30.0	4.95E-01	5.40E-01
B	30.5	4.96E-01	5.05E-01
C	31.7	5.25E-01	5.23E-01
D	33.2	0.47234	0.50421
E	35.4	0.46055	0.48352
F	38.3	0.40634	0.41586
G	41.7	0.10655	0.11048
H	44.5	0.0046552	0.0049655
I	46.8	0	0
J	48.3	0	0
K	49.5	0	0
L	50.0	0	0

Protein purified on 7/17/2023 and assayed on 7/19/2023.
Data uploaded by carterahlstedt and last updated on 2023-07-20 11:04:31.

Design Data

Rosetta Score for I170Y: 6.697

Citations

The unpublished data above were collected by students from Taylor University. For inquiries, please contact Daniel Kaluka: <daniel_kaluka@taylor.edu>.