Raw Data for BglB Variant N404E

Expression Data

SDS-PAGE
 0.466

Kinetic Assay Data

slope of N404E A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.74E-02 1.60E-02 1.59E-02
B 25.00 1.27E-02 1.31E-02 1.27E-02
C 8.33 1.16E-02 1.27E-02 1.07E-02
D 2.78 7.50E-03 6.20E-03 7.40E-03
E 0.93 4.90E-03 5.15E-03 5.90E-03
F 0.31 0 0 0
G 0.10 0 0 0
H 0.03 0 0 0
 0.466      10×

Protein purified on 7/12/23 and assayed on 7/13/23.
Data uploaded by kshows and last updated on 2023-07-13 19:50:36.


Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.



Thermostability Assay Data

slope of N404E A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00E+00 0.00E+00
B 48.3 4.00E-04 7.00E-04 0.00E+00
C 45.7 0.00E+00 0.00E+00
D 42.4 1.70E-03 1.70E-03 1.30E-03
E 37.7 9.90E-03 7.60E-03 8.10E-03
F 33.6 9.70E-03 8.80E-03 1.04E-02
G 31.3 8.30E-03 9.20E-03 8.30E-03
H 30.0 8.70E-03 9.90E-03 9.90E-03

Protein purified on 7/12/23 and assayed on 7/13/23.
Data uploaded by kshows and last updated on 2023-07-13 20:19:50.


Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.



Design Data

3.9


Citations

The unpublished data above were collected by students from Virginia State University. For inquiries, please contact Kate Shows: <kshows@vsu.edu>.