Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.652

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	4.88E-02		4.67E-02
	B	25.00	4.34E-02	3.86E-02	4.05E-02
	C	8.33	4.19E-02	3.98E-02	3.41E-02
	D	2.78	1.96E-02	2.11E-02	2.51E-02
	E	0.93	1.74E-02	1.66E-02	1.54E-02
	F	0.31	0		0
	G	0.10
	H	0.03

Yield (mg/mL): 0.652     Dilution Factor:  100×

Download these data

Protein purified on 7/12/2023 and assayed on 7/13/2023.
Data uploaded by kaschilling and last updated on 2023-07-13 19:48:53.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0
	B	48.3
	C	45.7	1.30E-03	1.00E-03	1.30E-03
	D	42.4	1.74E-02	1.41E-02	1.56E-02
	E	37.7		5.04E-02
	F	33.6		5.34E-02
	G	31.3	5.05E-02	4.31E-02	5.47E-02
	H	30.0	4.98E-02	5.24E-02	4.98E-02

Download these data

Protein purified on 7/12/2023 and assayed on 7/13/2023.
Data uploaded by kaschilling and last updated on 2023-07-13 19:56:52.

Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Kate Schilling: <katherine.schilling@yale.edu>.