D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H178N

Expression Data

Yield (mg/mL): 0.85098

Kinetic Assay Data

**slope of H178N A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.1559		0.14845
B	25.00	0.12703	0.12641	0.12145
C	8.33	0.091034	0.10045	0.088862
D	2.78	0.052655	0.052655	0.055345
E	0.93	0.024724	0.024621	0.024103
F	0.31	0.0088966	0.0096207	0.0097241
G	0.10	0.0038276	0.0036207	0.0036207
H	0.03	0	0	0

Yield (mg/mL): 0.85098 Dilution Factor: 100×

Protein purified on 7/17/23 and assayed on 7/18/23.
Data uploaded by annikabennett and last updated on 2023-07-21 10:27:44.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.34E-01	3.66E-01	3.54E-01
B	25.00	2.75E-01	2.88E-01	3.00E-01
C	8.33	2.20E-01	2.30E-01	2.12E-01
D	2.78	1.26E-01	1.29E-01	1.29E-01
E	0.93	5.72E-02	5.99E-02	5.76E-02
F	0.31	2.19E-02	2.37E-02	2.21E-02
G	0.10	8.03E-03	8.28E-03	7.76E-03
H	0.03	0	0	0

Yield (mg/mL): 1.638 Dilution Factor: 100×

Protein purified on 7/17/2023 and assayed on 7/18/2023.
Data uploaded by carterahlstedt and last updated on 2023-07-21 08:26:19.

Thermostability Assay Data

**slope of H178N A_{420 nm} (min⁻¹)**
	T (°C)	1	2
A	30.0	0.20307	0.21507
B	30.5	0.21134	0.20917
C	31.7	0.19728	0.18879
D	33.2	0.19045	0.192
E	35.4		0.18114
F	38.3	0.13334	0.14979
G	41.7		0.049552
H	44.5	0.0052759	0.0043448
I	46.8	0	0.0025862
J	48.3	0.0026897	0.0018621
K	49.5	0	0.0024828
L	50.0	0.0065172	0.0014483

Protein purified on 7/17/23 and assayed on 7/19/23.
Data uploaded by annikabennett and last updated on 2023-07-21 10:35:35.

Reference Data for WT

**slope of H178N A_{420 nm} (min⁻¹)**
	T (°C)	1	2
A	30.0	4.67E-01	4.79E-01
B	30.5	4.15E-01	3.08E-01
C	31.7	4.62E-01	4.63E-01
D	33.2	0.43976	0.40645
E	35.4		0.41514
F	38.3	0.33538	0.32938
G	41.7		0.099828
H	44.5	0.0054828	0.0056897
I	46.8	0.0013448	0.0024828
J	48.3	0.0028966	0.0010345
K	49.5	0.0032069	0.030724
L	50.0	0.0025862	0.0017586

Protein purified on 7/17/2023 and assayed on 7/19/2023.
Data uploaded by carterahlstedt and last updated on 2023-07-21 10:15:59.

Design Data

Rosetta Score for H178N: 3.25

Citations

The unpublished data above were collected by students from Taylor University. For inquiries, please contact Daniel Kaluka: <daniel_kaluka@taylor.edu>.