Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.638

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	3.34E-01	3.66E-01	3.54E-01
	B	25.00	2.75E-01	2.88E-01	3.00E-01
	C	8.33	2.20E-01	2.30E-01	2.12E-01
	D	2.78	1.26E-01	1.29E-01	1.29E-01
	E	0.93	5.72E-02	5.99E-02	5.76E-02
	F	0.31	2.19E-02	2.37E-02	2.21E-02
	G	0.10	8.03E-03	8.28E-03	7.76E-03
	H	0.03	0	0	0

Yield (mg/mL): 1.638     Dilution Factor:  100×

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Protein purified on 7/17/2023 and assayed on 7/18/2023.
Data uploaded by carterahlstedt and last updated on 2023-07-21 08:26:19.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2
	A	30.0	4.67E-01	4.79E-01
	B	30.5	4.15E-01	3.08E-01
	C	31.7	4.62E-01	4.63E-01
	D	33.2	0.43976	0.40645
	E	35.4		0.41514
	F	38.3	0.33538	0.32938
	G	41.7		0.099828
	H	44.5	0.0054828	0.0056897
	I	46.8	0.0013448	0.0024828
	J	48.3	0.0028966	0.0010345
	K	49.5	0.0032069	0.030724
	L	50.0	0.0025862	0.0017586

Download these data

Protein purified on 7/17/2023 and assayed on 7/19/2023.
Data uploaded by carterahlstedt and last updated on 2023-07-21 10:15:59.

Citations

The unpublished data above were collected by students from Taylor University. For inquiries, please contact Daniel Kaluka: <daniel_kaluka@taylor.edu>.