D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant M323I

Expression Data

Yield (mg/mL): 5.271

Kinetic Assay Data

**slope of M323I A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.80E-01	4.86E-01	4.92E-01
B	25.00	3.91E-01	4.08E-01	4.11E-01
C	8.33	2.95E-01	2.68E-01	2.61E-01
D	2.78	1.58E-01	1.23E-01	1.22E-01
E	0.93	6.22E-02	5.09E-02	5.60E-02
F	0.31	2.24E-02	1.68E-02	1.71E-02
G	0.10	7.23E-03	5.90E-03	7.70E-03
H	0.03	0.00E+00	0.00E+00	0.00E+00

Yield (mg/mL): 5.271 Dilution Factor: 100×

Protein purified on 7/6/23 and assayed on 7/7/23.
Data uploaded by alexasvf and last updated on 2023-07-27 00:40:16.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.87E-01	3.82E-01	3.95E-01
B	25.00	3.27E-01	3.23E-01	3.24E-01
C	8.33	2.11E-01	2.00E-01	2.06E-01
D	2.78	9.90E-02	1.02E-01	1.18E-01
E	0.93	4.35E-02	4.32E-02	4.98E-02
F	0.31	1.35E-02	1.45E-02	1.64E-02
G	0.10	5.40E-03	4.95E-03	5.80E-03
H	0.03		0	0

Yield (mg/mL): 3.88 Dilution Factor: 100×

Protein purified on 7/6/23 and assayed on 7/7/23.
Data uploaded by alexasvf and last updated on 2023-07-27 01:11:37.

Design Data

Rosetta Score for M323I: 6.406

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.